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KMID : 0364019950280050443
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Korean Journal of Thoracic and Cardiovascular Surgery
1995 Volume.28 No. 5 p.443 ~ p.446
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The Effect of Cyclosporine A on the expression of the Major Histocompatibility Complex Antigen Class II(MHC II)
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박국양/Douglas F. Larson
Jack G. Copeland
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Abstract
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The present study was designed to determine whether cyclosporine A inhibits Major Histocompatibility Complex Class Ⅱ antigen (MHC Ⅱ) expression in the allograft rat heart myocardium. In this rat allograft study we also tried to elucidate
whether
CSA
inhibits MHC Ⅱ in a dose dependent way. Hearts were isolated from LBN rats weighing 200~250 grams and heterotopically transplantated into the abdomen of weight-matched ACI rats.
@ES The ACI rats were randomly assigned to one of the three experimental groups according to cyclosporine dosage:
@EN {1} control [no CSA], n=6 {2} CSA 5mg/day, n=5 {3} CSA 10mg/day, n=5. The transplanted hearts were harvested 5 days postoperatively and analysed. MHC Ⅱ expression was detected by indirect immunoperoxidase staining and quantified via computer
image
anaysis system. The % positive area reading was obtained in each slide (50 areas per group) and compared to other groups. Significant differences were noted between three groups (P<0.05). We conclude that CSA inhibits MHC Ⅱ in heterotopically
transplanted allograft rat heart in a dose dependent way.
(Korean J Thorac Cardiovasc Surg 1995;28:443-6)
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